Introduction: The development of sonication over the past ̃ years has increased the sensitivity of conventional cultures for the diagnosis of Prosthetic Joint Infections (PJI). However, the development of a new kit, designed ̃ specifically for the diagnosis of IPAs could be a significant breakthrough in this field.

Methods: all prostheses removed from patients between May 2014 and May 2015 were sent to
culture using a processing protocol that includes implant sonication. In addition, 180 microliters of the sonication liquid was used to perform multiplex PCR (Unyvero i60®).
A comparison of the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) was performed.
(VPN). The study was approved by the Clinical Research Ethics Committee.
Results: 88 prostheses from 68 patients were analyzed (1.29 prostheses/patient). The prostheses studied
were knees (n = 55), total hip (n = 26), partial hip (n = 5), and shoulder (n = 2). Twenty-nine patients were diagnosed with IPA (15 chronic, 12 acute and 2 hematogenous). In 24 cases, the result was
The PCR test was positive, with all but 1 of these being from patients with IPA criteria. In addition, 9 resistance mechanisms were detected in 5 samples. The Unyvero i60® system showed slight results.
superior to culture in both specificity and PPV.

Conclusions: The Unyvero i60® system may have a role in the rapid diagnosis of IPA due to its high specificity and PPV. However, despite these results, culture should be performed to detect organisms not detected by the system.

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